Tris (hydroxymethyl) aminoomethane

☑ Tris buffer ba wai kawai ana amfani da shi azaman kaushi ga acid nucleic da proteins ba, har ma yana da amfani mai mahimmanci.An yi amfani da Tris don haɓakar kristal sunadaran a ƙarƙashin yanayin pH daban-daban.
☑ Za a iya amfani da ƙananan ƙarfin ionic na Tris buffer don samar da matsakaicin fiber na lamin a cikin C. elegans.
☑ Tris kuma yana daya daga cikin manyan abubuwan da ke cikin buffer electrophoresis protein.
☑ Bugu da kari, Tris shine matsakaici don shirye-shiryen surfactants, vulcanization accelerators da wasu magunguna.Hakanan ana amfani da Tris azaman ma'aunin titration.
☑Trimethylaminomethane ana amfani dashi sosai a cikin m metabolism da kuma numfashi acidemia.Yana da buffer alkaline kuma yana da sakamako mai kyau na buffer akan metabolism acidosis da halayen aikin enzyme.
☑Ana amfani da Tris akai-akai azaman buffer nazarin halittu, kuma ƙimar pH ɗin sa shine 6.8, 7.4, 8.0, 8.8.Tsarin tsarinsa, ƙimar pH yana canzawa sosai tare da zafin jiki.Gabaɗaya magana, ƙimar pH tana raguwa da 0.03 lokacin da zafin jiki ya ƙaru da digiri ɗaya.Ana amfani da Tris sosai wajen shirya maganin buffer a cikin nazarin halittu da gwaje-gwajen ilimin halitta.Misali, duka TAE da tbe buffers (an yi amfani da su don rushewar acid nucleic) da aka saba amfani da su a gwaje-gwajen sinadarai suna buƙatar Tris.Domin ya ƙunshi ƙungiyoyin amino, yana iya amsawa da aldehydes.
☑ Tris tushe ne mai rauni, kuma PKA ɗin sa shine 8.1 a zazzabi na ɗaki (25 ℃).Dangane da ka'idar buffer, ingantaccen kewayon buffer na Tris yana tsakanin 7.0 da 9.2.Ƙimar pH na maganin ruwa na Tris tushe shine kusan 10.5.Gabaɗaya, ana ƙara acid hydrochloric don daidaita ƙimar pH zuwa ƙimar da ake so don samun maganin buffer tare da wannan ƙimar pH.A lokaci guda, ya kamata mu kula da tasirin zafin jiki akan pKa na Tris.Saboda Tris buffer shine raunin alkaline mai rauni, DNA za ta rushe a cikin irin wannan bayani don inganta narkewar ta.Sau da yawa mutane suna ƙara EDTA cikin buffer na Tris hydrochloric acid don yin "Te buffer", wanda ake amfani dashi don daidaitawar DNA da adanawa.Idan an maye gurbin acid acid na daidaita darajar pH da acetic acid, ana samun "Tae buffer" (Tris / acetate / EDTA), kuma ana samun "tbe buffer" (Tris / borate / EDTA) ta maye gurbin shi da boric acid. .Ana amfani da waɗannan buffer guda biyu a gwaje-gwajen electrophoresis na nucleic acid.